Plasmid profile analysis of Portuguese Borrelia lusitaniae strains.
Vitorino L, Margos G, Zé-Zé L, Kurtenbach K, Collares-Pereira M
Ticks Tick Borne Dis 2010 09; 1 (3): 125-8
Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one
isolated from a human patient and the other one from an Ixodes ricinus
tick, were obtained by pulsed-field gel electrophoresis to evaluate the
plasmid diversity in each strain. Overall, a maximum of 6 plasmids were
detected that ranged from 19kb to 76kb, revealing completely different
plasmid profiles from those previously described for other genospecies
of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis.
The plasmid location of the ospA gene was investigated by hybridization,
allowing its allocation to the plasmid of 70kb instead of the 54kb
linear plasmid described for B. burgdorferi sensu stricto strains.
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