Identification of Anaplasma spp. Rickettsia Isolated from Horses from
Clinical Disease Cases in Poland.
Adaszek L, Winiarczyk S
Zoonoses Public Health 2011 02 1
This study was aimed at determining the cause of the diseases in five
horses exhibiting symptoms of fever, joint oedema and ataxia and
thrombocytopenia. The PCR technique revealed the presence in the blood
of 16S RNA Anaplasma/Ehrlichia spp. genetic material. DNA amplification
with primers EHR 521 and EHR 747 gave a product with a size of 247 bp.
The sequence of the PCR product obtained showed a 97.6-99.6% similarity
with a sequence of a fragment of 16S RNA Anaplasma phagocytophilum, gene
number EU 090186 from GenBank. Intravenous administration of
oxytetracycline at a dose of 8 mg/kg of body mass for 7 days resulted in
a gradual recovery.
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