Featured Post

Nutrition and Hormonal Balance

  Good Morning,  Nutrition and Hormonal Balance As an acupuncturist in the area of fertility, I realize tha...

Subscribe Updates via email

Subscribe Updates via email

Enter your email address:

Wednesday, February 23, 2011

[AlternativeAnswers] Large differences between test strategies for the detection of anti-Borrelia ant


Eur J Clin Microbiol Infect Dis. 2011 Jan 27; [Epub ahead of print]

Large differences between test strategies for the detection of
antibodies are revealed by comparing eight ELISAs and five immunoblots.

Ang CW, Notermans DW, Hommes M, Simoons-Smit AM, Herremans T.

VUMC, Amsterdam, The Netherlands,w.ang@vumc.nl.

We investigated the influence of assay choice on the results in a two-tier
testing algorithm for the detection of anti-Borrelia antibodies.

Eighty-nine serum samples from clinically well-defined patients were tested
in eight different enzyme-linked immunosorbent assay (ELISA) systems based
on whole-cell antigens, whole-cell antigens supplemented with VlsE and
assays using exclusively recombinant proteins.

A subset of samples was tested in five immunoblots: one whole-cell blot,
whole-cell blot supplemented with VlsE and three recombinant blots.

The number of IgM- and/or IgG-positive ELISA results in the group of
patients suspected of Borrelia infection ranged from 34 to 59%.

The percentage of positives in cross-reactivity controls ranged from 0 to

Comparison of immunoblots yielded large differences in inter-test agreement
and showed, at best, a moderate agreement between tests.

***Remarkably, some immunoblots gave positive results in samples that had
been tested negative by all eight ELISAs.***

The percentage of positive blots following a positive ELISA result depended
heavily on the choice of ELISA-immunoblot combination.

We conclude that the assays used to detect anti-Borrelia antibodies have
widely divergent sensitivity and specificity.

The choice of ELISA-immunoblot combination severely influences the number
positive results, making the exchange of test results between laboratories
with different methodologies hazardous.

PMID: 21271270 [PubMed - as supplied by publisher]

[Non-text portions of this message have been removed]

Recent Activity:
Peacefulmind.com Sponsors Alternative Answers-

HEALING NATURALLY- Learn preventative and curative measure to take for many ailments at:


-To INVITE A FRIEND to our healing community, copy and paste this address in an email to them:


To ADD A LINK, RESOURCE, OR WEBSITE to Alternative Answers please Go to:


Community email addresses:
  Post message: AlternativeAnswers@yahoogroups.com
  Subscribe:    AlternativeAnswers-subscribe@yahoogroups.com
  Unsubscribe:  AlternativeAnswers-unsubscribe@yahoogroups.com
  List owner:   AlternativeAnswers-owner@yahoogroups.com
Shortcut URL to this page:


No comments: