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Thursday, May 19, 2011

[AlternativeAnswers] ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlic

 




Am J Vet Res. 2010 Dec;71(12):1443-50.

Performance of a commercially available in-clinic ELISA for the
detection of antibodies against Anaplasma phagocytophilum, Ehrlichia
canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs.

Chandrashekar R, Mainville CA, Beall MJ, O'Connor T, Eberts MD, Alleman
AR, Gaunt SD, Breitschwerdt EB.

Immunoassay R&D, IDEXX Laboratories, 1 IDEXX Dr, Westbrook, ME 04092,
USA. _chandra@idexx.com_ (mailto:chandra@idexx.com)

Abstract

OBJECTIVE: To evaluate the sensitivity and specificity of a commercially
available in-clinic ELISA for detection of heartworm infection and
tick-borne diseases in dogs.

SAMPLE POPULATION: 846 serum, plasma, or blood samples obtained from dogs.

PROCEDURES: Samples were evaluated via the in-clinic ELISA to detect
antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and
Borrelia burgdorferi and Dirofilaria immitis (heartworm) antigen. True
infection or immunologic status of samples was assessed by use of
results of necropsy, an antigen assay for D immitis, and
immunofluorescence assay or western blot analysis for antibodies against
B burgdorferi, E canis, and A phagocytophilum.

RESULTS: Sensitivity and specificity of the in-clinic ELISA for
detection of heartworm antigen (99.2% and 100%, respectively),
antibodies against B burgdorferi (98.8% and 100%, respectively), and
antibodies against E canis (96.2% and 100%, respectively) were similar
to results for a similar commercial ELISA. In samples obtained from dogs
in the northeast and upper Midwest of the United States, sensitivity and
specificity of the in-clinic ELISA for antibodies against Anaplasma spp
were 99.1% and 100%, respectively, compared with results for an
immunofluorescence assay. Samples from 2 dogs experimentally infected
with the NY18 strain of A phagocytophilum were tested by use of the
in-clinic ELISA, and antibodies against A phagocytophilum were detected
by 8 days after inoculation. Antibodies against Anaplasma platys in
experimentally infected dogs cross-reacted with the A phagocytophilum
analyte. Coinfections were identified in several of the canine serum
samples.

CONCLUSIONS AND CLINICAL RELEVANCE: The commercially available in-clinic
ELISA could be used by veterinarians to screen dogs for heartworm
infection and for exposure to tick-borne pathogens.

PMID: 21117995 [PubMed - indexed for MEDLINE]

[Non-text portions of this message have been removed]

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