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Monday, July 11, 2011

[AlternativeAnswers] Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolates from Northeastern

 


_http://www.liebertonline.com/doi/abs/10.1089/vbz.2009.0195_
(http://www.liebertonline.com/doi/abs/10.1089/vbz.2009.0195)

Vector-Borne and Zoonotic Diseases

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolates from
Northeastern China

To cite this article:
Chen-Yi Chu, Bao-Gui Jiang, Jing He, Yan Gao, Pan-He Zhang, Xiao-Ming
Wu, Wen-Yi Zhang, Hua Shi, Ha-Sen Gaowa, Jian-Bo Wang, Janet E. Foley,
Wei Liu and Wu-Chun Cao.

Vector-Borne and Zoonotic Diseases. July 2011, 11(7): 877-882.
doi:10.1089/vbz.2009.0195.

Published in Volume: 11 Issue 7: July 7, 2011

Online Ahead of Print: December 13, 2010

Abstract

Thirty-two strains of Borrelia burgdorferi sensu lato were isolated from
Ixodes persulcatus ticks collected from northeastern China from May to
June in 2004 and 2005. Restriction fragment length polymorphism (RFLP)
analysis and sequence analysis of 5S-23S rRNA intergenic spacer revealed
that 29 (90.6%) belonged to Borrelia garinii, demonstrating B, C, and a
unique pattern. The remaining three isolates (9.4%) were Borrelia
afzelii with pattern D. The phylogenetic analysis based on 5S-23S rRNA
intergenic spacer showed that B. garinii and B. afzelii genospecies
clustered into two separate lineages. B. garinii strains were classified
into three different branches: All the strains with RFLP pattern C were
in the same branch, strain VH10 with a unique RFLP pattern clustered
with strains VH9 and MDH2 with pattern B, and the rest of the strains
with pattern B constitute another branch. These findings demonstrate the
genetic diversity of B. burgdorferi sensu lato isolates from
northeastern China.

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